Quantitative and holistic superresolution live-cell imaging: from structured illumination microscopy to the sparse deconvolution

Here we present an overview of our recent works in live-cell superresolution (SR) microscopy. The first is a structured illumination microscopy technique based on the continuity of biological structures embedded in Hessian matrices (Hessian-SIM), which significantly reduces the photon dosage required for SR microscopy while suppressing reconstruction artifacts induced by random noise. Next, we developed a dual-mode microscopy technique that combines SIM with label-free three-dimensional optical diffraction tomography to enable holistic SR imaging. To further push the resolution limit of live-cell SR imaging, we developed a two-step iterative deconvolution algorithm based on continuity and sparsity of fluorescence signals (Sparse deconvolution), which extends resolutions beyond the physical limits of optical systems. To make live-cell SIM microscopy more quantitative, we proposed a physical model-based background removal method (BF-SIM). Finally, we develop a rolling Fourier ring correlation method to non-biasedly evaluate reconstruction uncertainty of the SR images.

The SCALE lunch seminar will take place in a hybrid form. All interested are invited to attend in person at the lecture hall of the MPI-BP with coffee and a light lunch afterwards. At the same time, the lecture will also be streamed online via zoom. The zoom link will be sent by e-mail to the SCALE mailing list. To subscribe to the mailing list, please send an e-mail to scale-office@uni-frankfurt.de.